你也可以使用ctypes来进入Windows API，避免大量的pywin32包。这是我使用的方法(请原谅我的风格不好，但我的想法是这样的):

import ctypes

# Get required functions, strcpy..
strcpy = ctypes.cdll.msvcrt.strcpy
ocb = ctypes.windll.user32.OpenClipboard    # Basic clipboard functions
ecb = ctypes.windll.user32.EmptyClipboard
gcd = ctypes.windll.user32.GetClipboardData
scd = ctypes.windll.user32.SetClipboardData
ccb = ctypes.windll.user32.CloseClipboard
ga = ctypes.windll.kernel32.GlobalAlloc    # Global memory allocation
gl = ctypes.windll.kernel32.GlobalLock     # Global memory Locking
gul = ctypes.windll.kernel32.GlobalUnlock
GMEM_DDESHARE = 0x2000

def Get():
  ocb(None) # Open Clip, Default task

  pcontents = gcd(1) # 1 means CF_TEXT.. too lazy to get the token thingy...

  data = ctypes.c_char_p(pcontents).value

  #gul(pcontents) ?
  ccb()

  return data

def Paste(data):
  ocb(None) # Open Clip, Default task

  ecb()

  hCd = ga(GMEM_DDESHARE, len(bytes(data,"ascii")) + 1)

  pchData = gl(hCd)

  strcpy(ctypes.c_char_p(pchData), bytes(data, "ascii"))

  gul(hCd)

  scd(1, hCd)

  ccb()

看起来您需要将win32clipboard添加到站点包中。它是pywin32包的一部分

这是雾化器改进后的答案。

注意2次update()调用和它们之间200ms的延迟。它们可以保护由于剪贴板状态不稳定而冻结的应用程序:

from Tkinter import Tk
import time     

r = Tk()
r.withdraw()
r.clipboard_clear()
r.clipboard_append('some string')

r.update()
time.sleep(.2)
r.update()

r.destroy()

小部件还具有名为.clipboard_get()的方法，该方法返回剪贴板的内容(除非根据剪贴板中的数据类型发生某种错误)。

在这个错误报告中提到了clipboard_get()方法: http://bugs.python.org/issue14777

奇怪的是，在我通常参考的常见(但非官方的)在线TkInter文档源中并没有提到这种方法。

除了Mark Ransom使用ctypes的回答之外: 这并不适用于(所有?)x64系统，因为句柄似乎被截断为int-size。 显式定义参数和返回值有助于克服这个问题。

import ctypes
import ctypes.wintypes as w

CF_UNICODETEXT = 13

u32 = ctypes.WinDLL('user32')
k32 = ctypes.WinDLL('kernel32')

OpenClipboard = u32.OpenClipboard
OpenClipboard.argtypes = w.HWND,
OpenClipboard.restype = w.BOOL

GetClipboardData = u32.GetClipboardData
GetClipboardData.argtypes = w.UINT,
GetClipboardData.restype = w.HANDLE

EmptyClipboard = u32.EmptyClipboard
EmptyClipboard.restype = w.BOOL

SetClipboardData = u32.SetClipboardData
SetClipboardData.argtypes = w.UINT, w.HANDLE,
SetClipboardData.restype = w.HANDLE

CloseClipboard = u32.CloseClipboard
CloseClipboard.argtypes = None
CloseClipboard.restype = w.BOOL

GHND = 0x0042

GlobalAlloc = k32.GlobalAlloc
GlobalAlloc.argtypes = w.UINT, w.ctypes.c_size_t,
GlobalAlloc.restype = w.HGLOBAL

GlobalLock = k32.GlobalLock
GlobalLock.argtypes = w.HGLOBAL,
GlobalLock.restype = w.LPVOID

GlobalUnlock = k32.GlobalUnlock
GlobalUnlock.argtypes = w.HGLOBAL,
GlobalUnlock.restype = w.BOOL

GlobalSize = k32.GlobalSize
GlobalSize.argtypes = w.HGLOBAL,
GlobalSize.restype = w.ctypes.c_size_t

unicode_type = type(u'')

def get():
    text = None
    OpenClipboard(None)
    handle = GetClipboardData(CF_UNICODETEXT)
    pcontents = GlobalLock(handle)
    size = GlobalSize(handle)
    if pcontents and size:
        raw_data = ctypes.create_string_buffer(size)
        ctypes.memmove(raw_data, pcontents, size)
        text = raw_data.raw.decode('utf-16le').rstrip(u'\0')
    GlobalUnlock(handle)
    CloseClipboard()
    return text

def put(s):
    if not isinstance(s, unicode_type):
        s = s.decode('mbcs')
    data = s.encode('utf-16le')
    OpenClipboard(None)
    EmptyClipboard()
    handle = GlobalAlloc(GHND, len(data) + 2)
    pcontents = GlobalLock(handle)
    ctypes.memmove(pcontents, data, len(data))
    GlobalUnlock(handle)
    SetClipboardData(CF_UNICODETEXT, handle)
    CloseClipboard()

#Test run
paste = get
copy = put
copy("Hello World!")
print(paste())

可以使用pyperclip -跨平台剪贴板模块。或施乐类似的模块，除了需要win32 Python模块才能在Windows上工作。