现在我在用ImageMagick。我将帧保存为PNG文件，然后从Python中调用ImageMagick的convert.exe来创建一个动画GIF。这种方法的好处是我可以为每一帧单独指定帧持续时间。不幸的是，这取决于ImageMagick安装在机器上。他们有一个Python包装器，但它看起来很糟糕，不受支持。还在考虑其他建议。

使用windows7, python2.7, opencv 3.0，以下工作为我:

import cv2
import os

vvw           =   cv2.VideoWriter('mymovie.avi',cv2.VideoWriter_fourcc('X','V','I','D'),24,(640,480))
frameslist    =   os.listdir('.\\frames')
howmanyframes =   len(frameslist)
print('Frames count: '+str(howmanyframes)) #just for debugging

for i in range(0,howmanyframes):
    print(i)
    theframe = cv2.imread('.\\frames\\'+frameslist[i])
    vvw.write(theframe)

我偶然发现了PIL的ImageSequence模块，它提供了更好(更标准)的GIF动画。这次我也使用了Tk的after()方法，这比time.sleep()更好。

from Tkinter import * 
from PIL import Image, ImageTk, ImageSequence

def stop(event):
  global play
  play = False
  exit() 

root = Tk()
root.bind("<Key>", stop) # Press any key to stop
GIFfile = {path_to_your_GIF_file}
im = Image.open(GIFfile); img = ImageTk.PhotoImage(im)
delay = im.info['duration'] # Delay used in the GIF file 
lbl = Label(image=img); lbl.pack() # Create a label where to display images
play = True;
while play:
  for frame in ImageSequence.Iterator(im):
    if not play: break 
    root.after(delay);
    img = ImageTk.PhotoImage(frame)
    lbl.config(image=img); root.update() # Show the new frame/image

root.mainloop()

下面是如何使用PIL (install with: pip install Pillow):

import glob
import contextlib
from PIL import Image

# filepaths
fp_in = "/path/to/image_*.png"
fp_out = "/path/to/image.gif"

# use exit stack to automatically close opened images
with contextlib.ExitStack() as stack:

    # lazily load images
    imgs = (stack.enter_context(Image.open(f))
            for f in sorted(glob.glob(fp_in)))

    # extract  first image from iterator
    img = next(imgs)

    # https://pillow.readthedocs.io/en/stable/handbook/image-file-formats.html#gif
    img.save(fp=fp_out, format='GIF', append_images=imgs,
             save_all=True, duration=200, loop=0)

查看文档:https://pillow.readthedocs.io/en/stable/handbook/image-file-formats.html#gif

要创建视频，你可以使用opencv，

#load your frames
frames = ...
#create a video writer
writer = cvCreateVideoWriter(filename, -1, fps, frame_size, is_color=1)
#and write your frames in a loop if you want
cvWriteFrame(writer, frames[i])

我建议不要使用visvis的images2gif，因为它有PIL/Pillow的问题，并且没有积极维护(我应该知道，因为我是作者)。

相反，请使用imageio，它是为解决这个问题而开发的，并打算保留。

快速而肮脏的解决方案:

import imageio
images = []
for filename in filenames:
    images.append(imageio.imread(filename))
imageio.mimsave('/path/to/movie.gif', images)

对于较长的电影，使用流媒体方法:

import imageio
with imageio.get_writer('/path/to/movie.gif', mode='I') as writer:
    for filename in filenames:
        image = imageio.imread(filename)
        writer.append_data(image)