这是我的2美分，不管它是否值得;)

def indexLines(filename, lines=[2,4,6,8,10,12,3,5,7,1]):
    fp   = open(filename, "r")
    src  = fp.readlines()
    data = [(index, line) for index, line in enumerate(src) if index in lines]
    fp.close()
    return data


# Usage below
filename = "C:\\Your\\Path\\And\\Filename.txt"
for line in indexLines(filename): # using default list, specify your own list of lines otherwise
    print "Line: %s\nData: %s\n" % (line[0], line[1])

file = '/path/to/file_to_be_read.txt'
with open(file) as f:
    print f.readlines()[26]
    print f.readlines()[30]

使用with语句打开文件，打印第26行和第30行，然后关闭文件。简单!

不要使用阅读线!

我的解决方案是:

with open(filename) as f:
    specify = [26, 30]
    results = list(
        map(lambda line: line[1],
            filter(lambda line: line[0] in specify,
                   enumerate(f))
            )
    )

对6.5G文件进行如下测试:

import time

filename = 'a.txt'
start = time.time()
with open(filename, 'w') as f:
    for i in range(10_000_000):
        f.write(f'{str(i)*100}\n')       
end1 = time.time()

with open(filename) as f:
    specify = [26, 30]
    results = list(
        map(lambda line: line[1],
            filter(lambda line: line[0] in specify,
                   enumerate(f))
            )
    )
end2 = time.time()
print(f'write time: {end1-start}')
print(f'read time: {end2-end1}')
# write time: 14.38945460319519
# read time: 8.380386352539062

文件对象有一个.readlines()方法，它将为您提供文件内容的列表，每个列表项一行。在此之后，您可以使用普通的列表切片技术。

http://docs.python.org/library/stdtypes.html#file.readlines

这个怎么样:

>>> with open('a', 'r') as fin: lines = fin.readlines()
>>> for i, line in enumerate(lines):
      if i > 30: break
      if i == 26: dox()
      if i == 30: doy()

你可以用已经有人提到过的语法很简单地做到这一点，但这是迄今为止最简单的方法:

inputFile = open("lineNumbers.txt", "r")
lines = inputFile.readlines()
print (lines[0])
print (lines[2])