查看File对象的.read()方法:

http://docs.python.org/2/tutorial/inputoutput.html#methods-of-file-objects

你可以这样做:

concat = ""
for file in files:
    concat += open(file).read()

或者更“优雅”的python方式:

concat = ''.join([open(f).read() for f in files])

根据这篇文章，http://www.skymind.com/~ocrow/python_string/也将是最快的。

UNIX命令有什么问题?(假设你不是在Windows上工作):

Ls | xargs cat | tee output.txt完成这项工作(如果你想要，你可以从python用subprocess调用它)

使用shutil.copyfileobj。

它会自动读取输入文件的块为您，这是更有效的读取输入文件，即使一些输入文件太大，无法装入内存也能工作:

import shutil

with open('output_file.txt','wb') as wfd:
    for f in ['seg1.txt','seg2.txt','seg3.txt']:
        with open(f,'rb') as fd:
            shutil.copyfileobj(fd, wfd)

def concatFiles():
    path = 'input/'
    files = os.listdir(path)
    for idx, infile in enumerate(files):
        print ("File #" + str(idx) + "  " + infile)
    concat = ''.join([open(path + f).read() for f in files])
    with open("output_concatFile.txt", "w") as fo:
        fo.write(path + concat)

if __name__ == "__main__":
    concatFiles()

outfile.write(infile.read()) # time: 2.1085190773010254s
shutil.copyfileobj(fd, wfd, 1024*1024*10) # time: 0.60599684715271s

一个简单的基准测试表明，shutil的性能更好。

如果目录中有很多文件，那么glob2可能是生成文件名列表的更好选择，而不是手工编写它们。

import glob2

filenames = glob2.glob('*.txt')  # list of all .txt files in the directory

with open('outfile.txt', 'w') as f:
    for file in filenames:
        with open(file) as infile:
            f.write(infile.read()+'\n')