试试这个:

[[UINavigationBar appearance] setBackgroundImage: [UIImage new]  
                                   forBarMetrics: UIBarMetricsDefault];

[UINavigationBar appearance].shadowImage = [UIImage new];

下图有解释(iOS7导航栏):

检查这个SO问题: iOS7 -改变UINavigationBar的边框颜色

在Swift 3中，我们就是这样做的

对于任何视图控制器:

navigationBar.shadowImage = UIImage()
setBackgroundImage(UIImage(), for: .default)

对于整个应用程序:

UINavigationBar.appearance().setBackgroundImage(UIImage(),barMetrics: .Default)
UINavigationBar.appearance().shadowImage = UIImage()

编写自己的初始化式:D

import Foundation
import UIKit

extension UINavigationController {
    convenience init(rootViewController : UIViewController, hidesShadow : Bool) {
        self.init(rootViewController : rootViewController)
        self.navigationBar.setValue(hidesShadow, forKey: "hidesShadow")
        if hidesShadow {
            self.extendedLayoutIncludesOpaqueBars = true
            self.navigationBar.isTranslucent = false 
        }
    }
}

快速做这件事的方法:

UINavigationBar.appearance().setBackgroundImage(UIImage(), for: .any, barMetrics: .default)
UINavigationBar.appearance().shadowImage = UIImage()

从iOS 13开始，就有一个系统API来设置或删除阴影

UIKit使用shadowImage和shadowColor属性来确定阴影的 外观。当shadowImage为nil时，该工具条显示默认的着色阴影 根据shadowColor属性中的值。如果shadowColor为nil或 包含clearColor颜色，条显示没有阴影。

    let appearance = UINavigationBarAppearance()
    appearance.shadowImage = nil
    appearance.shadowColor = nil
    navigationController.navigationBar.standardAppearance = appearance

https://developer.apple.com/documentation/uikit/uibarappearance/3198009-shadowimage

想把Serhii回答的Swift版本加进去。我用下面的代码创建了一个uibareextension .swift:

import Foundation
import UIKit

extension UINavigationBar {
    func hideBottomHairline() {
        self.hairlineImageView?.isHidden = true
    }

    func showBottomHairline() {
        self.hairlineImageView?.isHidden = false
    }
}

extension UIToolbar {
    func hideBottomHairline() {
        self.hairlineImageView?.isHidden = true
    }

    func showBottomHairline() {
        self.hairlineImageView?.isHidden = false
    }
}

extension UIView {
    fileprivate var hairlineImageView: UIImageView? {
        return hairlineImageView(in: self)
    }

    fileprivate func hairlineImageView(in view: UIView) -> UIImageView? {
        if let imageView = view as? UIImageView, imageView.bounds.height <= 1.0 {
            return imageView
        }

        for subview in view.subviews {
            if let imageView = self.hairlineImageView(in: subview) { return imageView }
        }

        return nil
    }
}